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The laboratory is located in the ICA building (5th floor) and supports research on the biogeochemistry of a variety of systems, but particular attention is focused on aquatic systems. The laboratory has the potential to perform a wide range of soil, vegetation and water chemistry analyses commonly associated with elemental cycling research. Equipment includes chemical instrumentation for quantifying different analyte concentrations, drying ovens, incubators, balances, a muffle furnace, filtering apparatus, vacuum hoods, sampling devices, PC's and printers. |
To contact with the Lab for any question, support or analysis request, please e-mail to José Luis Ayala (jlayala@mncn.csic.es)
Available instrumental in the laboratory:
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1) Perkin Elmer 2400 Serie II for elemental analysis (CHNO)
2) Perkin Elmer Lambda 35 spectrophotometer 3) Shimadzu TOC-V CSH coupled with a TNM-1 for dissolved carbon and nitrogen analyses 4) SEAL Analytical AutoAnalyzer 3 5) Agilent Technologies 6890N gas chromatograph for CO2 and CH4 analysis. |
For field campaings the following equipment is also available:
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1) Niskin bottles
2) Crison MM40 pH and conducitivimeter 3) CTD SEABRIR SBE 19Plus 4) YSI oxymeter ProODO 5) YSI 6920 V2 multi-parameter sonde 6) Hach HQ40d multi-parameter sonde (oxygen and ORP) 7) FP101 Flowprobe Global Water sonde for water flow measurements 8) Furuno FE-450 Echosound 9) Portable infrared gas analyzer (IRGA) model 225 MK3 (ADC BioScientific) for leaf-level photosynthesis, transpiration, and stomatal conductance 10) AccuPAR LP-80 Ceptometer (Decagon Devices Inc.) for LAI measurements 11) Closed soil respiration chamber SRC-1 connected to an EGM-4 CO2 gas analyzer (PP-systems) 12) Soil moisture and temperature EC-10 and EC-T ECH2O probes (Decagon Devices Inc.) 13) Zodiac inflatable and portable boats |
Available analyses in the laboratory:
1) In water samples:
- Total organic carbon (TOC) in water samples, including particulate organic carbon (POC), dissolved organic carbon (DOC) and total nitrogen (TN)
- Ammonium, nitrite, nitrate, orthophosphate and total phosphorus (TP), following APHA (2005)
- Total solids (110 ºC), total mineral and organic matter (loss of ignition 550 ºC), suspended solids, carbonates-bicarbonates, clorides, sulfates, calcium, magnesium, sodium, potasium, silica, total, temporary and permanent hardness, following APHA (2005)
- Water colour (ʎ = 440 nm, in mg Pt/l)
- Chlorophyll-a (after methanol extraction, following Maker et al. (1980)
- Dissolved oxygen by the Winkler (tritation) method, following APHA (2005)
- Soil water content (gravimetric method)
- Elemental analysis of C, N and P (after acid digestion using ammonium persulfate/sulfuric acid)
- Nutrient extraction (nitrogen with CaCl2 and assimilable phosphorus with the Olsen method) for further analysis following previous description (see 3)
- Carbonates using the Bernard calcimeter
- Microbial biomass C, N and P by chloroform fumigation-incubation method (Brookes et al. 1985, Inubushi et al. 1991, Ivanoff et al. 1998, Witt et al. 2000, Truu et al. 2009, Sánchez-Carrillo et al. 2014) and then measured by the Shimadzu TOC-V analyzer (C and N) and by the SEAL autoanalyzer (P, after acid digestion; see above)
- Chlorophyll-(a+b) after methanol extraction and following Porra, Thompson and Kriedemann (1989)
- Elemental analysis of C, N and P (after acid digestion using ammonium persulfate/sulfuric acid)
References
APHA (2005) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington DC.
Marker AFH, Nusch EA, Rai H, Rieman B (1980) The measurement of photosynthetic pigments in freshwater and standardisation of methods: conclusions and recommendations. Archiv für Hydrobiologie 14: 91-106.
Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure MBNitrogen in soil. Soil Biology and Biochemistry 17: 837-842.
Inubushi K, Brookes PC, Jenkinson DS (1991) Soil MBC, N and ninhydrin-N in aerobic and anaerobic soils measured by the fumigation-extraction method. Soil Biology and Biochemistry 23:737–741
Ivanoff DB, Reddy KR, Robinson S (1998) Chemical fractionation of organic P in histosols. Soil Science 163:36-45
Witt C, Gaunt JL, Galicia CC, Ottow JCG, Neue H-U (2000) A rapid chloroform-fumigation ex-traction method for measuring soil MBCarbon and nitrogen in flooded rice soils. Biology and Fertility of Soils 30:510–519
Truu M, Juhanson J, Truu J (2009) Microbial biomass, activity and community composition in constructed wetlands. Science of Total Environment 407: 3958-3971
Sánchez-Carrillo S, Reddy KR, Inglett KS, Álvarez-Cobelas M, Sánchez-Andrés R (2014) Biogeochemical indicators of nutrient enrichment in wetlands: microbial response as a sensitive indicator of wetland eutrophication. En: AA Ansari, SS Gill (eds), Eutrophication: Causes, Consequences and Control Vol 2. Springer, Berlin. pp. 203-222.
Porra RJ, Thompson WA, Kriedemann PE (1989) Biochimica et Biophysica Acta 975: 384-394.
Marker AFH, Nusch EA, Rai H, Rieman B (1980) The measurement of photosynthetic pigments in freshwater and standardisation of methods: conclusions and recommendations. Archiv für Hydrobiologie 14: 91-106.
Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure MBNitrogen in soil. Soil Biology and Biochemistry 17: 837-842.
Inubushi K, Brookes PC, Jenkinson DS (1991) Soil MBC, N and ninhydrin-N in aerobic and anaerobic soils measured by the fumigation-extraction method. Soil Biology and Biochemistry 23:737–741
Ivanoff DB, Reddy KR, Robinson S (1998) Chemical fractionation of organic P in histosols. Soil Science 163:36-45
Witt C, Gaunt JL, Galicia CC, Ottow JCG, Neue H-U (2000) A rapid chloroform-fumigation ex-traction method for measuring soil MBCarbon and nitrogen in flooded rice soils. Biology and Fertility of Soils 30:510–519
Truu M, Juhanson J, Truu J (2009) Microbial biomass, activity and community composition in constructed wetlands. Science of Total Environment 407: 3958-3971
Sánchez-Carrillo S, Reddy KR, Inglett KS, Álvarez-Cobelas M, Sánchez-Andrés R (2014) Biogeochemical indicators of nutrient enrichment in wetlands: microbial response as a sensitive indicator of wetland eutrophication. En: AA Ansari, SS Gill (eds), Eutrophication: Causes, Consequences and Control Vol 2. Springer, Berlin. pp. 203-222.
Porra RJ, Thompson WA, Kriedemann PE (1989) Biochimica et Biophysica Acta 975: 384-394.